Figure 3.

Optimization of polymer-based cultures for single-cell HSC expansion

(A) Scheme of experimental setup.

(B) Percentage of colonies with ≥20% live cells (n = 5 experiments). Unpaired, two-tailed t test.

(C) Percentage of phenotypic HSC populations in live colonies cultured in PVA (n = 94)- and Soluplus (n = 155)-based media. Multiple Mann-Whitney tests with FDR correction.

(D) Schematic of split-clone transplantation.

(E and F) Donor PB chimerism (E) and lineage distribution (F) in 3 recipient groups transplanted with split clones. Numbers over graphs in (E) represent percentage of CD201⁺CD150⁺KSL cells in the transplanted clone (%) and the number of recipients (n). Secondary SCT was performed with the group showing highest chimerism, data shown in graph with gray axis.

(G) Left: ELDA output of HSC frequency calculation. Right: boxplot represents calculated reciprocal mean, upper, and lower limits of HSC frequency.

See also Figure S3 and Table S2.

Error bars represent SD. ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.