TABLE 9.
The IC 50 values of the existing inhibitors of ADAM17 on the Notch pathway in NSCLC cells.
| Agents | A549 | H460 | H520 |
|---|---|---|---|
| IC 50 on survivin | |||
| compound 2b | 1.47 ± 0.71 | 1.65 ± 0.42 | 1.26 ± 0.31 |
| pratastat | 1.95 ± 0.18 | 1.77 ± 0.40 | 1.55 ± 0.67 |
| terfenadine | 2.61 ± 0.44 | 1.81 ± 0.23 | 1.86 ± 0.66 |
| ZLDI-8 | 2.02 ± 0.93 | 1.93 ± 0.20 | 1.83 ± 0.35 |
The NSCLC, cells were cultured and treated for the indicated concentrations of the existing inhibitors (compound 2b, pratastat, terfenadine, ZLDI-8) of ADAM17. The cells were harvested for the qPCR, to examine the expression of Survivin, which is a typical downstream gene of Notch pathway. The inhibition rate of Survivin expression by different doses of the drug on NSCLC, cells was used to calculate the IC 50 values and ultimately reflect the inhibitory activity of the drug on the Notch pathway.