Fig. 4.

β3 integrin deletion in osteocytes leads to altered FA signaling pathway and abnormal cell morphology. (a) Western blotting analyses of FA signaling pathway proteins in cortical bone samples from control and cKO mice. Gapdh was used as a loading control. n ​= ​3 for each group. (b) Representative H&E staining images of tibiae bone sections of control and cKO mice. (c) Representative images from F-actin cytoskeleton staining of control and cKO radius bones. (d) Representative images from LCS staining of control and cKO femurs. (e–i) Quantitative analyses of osteocyte numbers, nuclear size, dendritic number per cell, the longest dendritic length in each cell and the lacunar size between control and cKO bone samples. n ​= ​5 for each group. Results are expressed as mean ​± ​standard deviation (s.d.). n. s. P ​> ​0.05; ∗p ​< ​0.05; ∗∗p ​< ​0.01; ∗∗∗p ​< ​0.001.