Table 1.
Primer sequences of genes used in quantitative real-time-PCR.
| Species | Gene | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|---|
| Zebrafish | ALP | CTGGTGGAAGGTGGACGCATTG | CGTGTCATACTCGCTTGTCAGGAG |
| RUNX2a | AGACTCCGACCTCACGACAACC | GGCAGCACCGAGCACAGAAAG | |
| SP7 | AGGCTTGCTAACACCAACTGGAAG | GGGAAACACTGGAGGTCTGGAAG | |
| OCN | GTCCTGATCTTCTGCTGCCTGATG | GTCACGCTTCACAAACACACCTTC | |
| CTSK | GTGCCTGATGACAGAGTGGGAAAG | AGCCGCATGATCCTTGGTTCTTC | |
| RANKL | ACACTCACGGTCTGAACCTCCTAC | ACACCTCTGCCCTGCTCCATG | |
| NFATC1 | TCTGCCATTGTTGCCGCCATC | TGTCTCGTCTCCACCTGGTTCTAC | |
| TRAF | GGTACAAACTCTGCCTGCGACTG | CCAGCACTGCCAGCCGAATG | |
| GAPDH | CGCTGGTGCTGGTATTGCTCTC | GCCATCAGGTCACATACACGGTTG | |
| Human | COL1 | AAAGATGGACTCAACGGTCTC | CATCGTGAGCCTTCTCTTGAG |
| RUNX2 | AGGCAGTTCCCAAGCATTTCATCC | TGGCAGGTAGGTGTGGTAGTGAG | |
| SP7 | CGGCAAGAGGTTCACTCGTTCG | TGGAGCAGAGCAGGCAGGTG | |
| OCN | AGGGCAGCGAGGTAGTGAAGAG | GCCGATGTGGTCAGCCAACTC | |
| Mouse | COL1 | GACAGGCGAACAAGGTGACAGAG | CAGGAGAACCAGGAGAACCAGGAG |
| RUNX2 | GATGATGACACTGCCACCTCTGAC | TGAGGGATGAAATGCTTGGGAACTG | |
| SP7 | GACTACCCACCCTTCCCTCACTC | TAGACACTAGGCAGGCAGTCAGAC | |
| OCN | CAAGCAGGAGGGCAATAAGGTAGTG | CGGTCTTCAAGCCATACTGGTCTG |
PCR, polymerase chain reaction; ALP, alkaline phosphatase; RUNX2a, runt-related transcription factor 2a; SP7, Sp7 transcription factor; OCN, osteocalcin; CTSK, cathepsin K; RANKL, receptor of nuclear factor-κB; NFATC1, nuclear factor of activated T cells 1; TRAF6, tumor necrosis trapping receptor associated factor 6; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; COL1, collagen type I