Table 2.
Primer sequences for the qPCR.
| Target gene1 | Primer sequence | Size (bp) | GenBank accession number |
|---|---|---|---|
| ZO-1 | Forward: 5′-GCCAACTGATGCTGAACCAA-3′ Reverse: 5′-GGGAGAGACAGGACAGGACT-3′ |
141 | XM_015278975 |
| Oocludin | Forward: 5′-GATGGACAGCATCAACGACC-3′ Reverse: 5′-CTTGCTTTGGTAGTCTGGGC-3′ |
142 | NM_205128.1 |
| Claudin-1 | Forward: 5′-CTGATTGCTTCCAACCAG-3′ Reverse: 5′-CAGGTCAAACAGAGGTACAAG-3′ |
140 | NM_001013611.2 |
| GAPDH | Forward: 5′-TGCTGCCCAGAACATCATCC-3′ Reverse: 5′-ACGGCAGGTCAGGTCAACAA-3′ |
142 | NM_204305.2 |
1
ZO-1: zona occludens-1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.