Table 2.

Summary of statistical analysis of the area of the axonal projections

a. Analysis of variance
Source	DF	Sum of squares	Mean square	F ratio	Prob > F
Model	7	70,043,555	10,006,222	51.0420
Error	96	18,819,747	196039.03
C. Total	103	88,863,302			<0.0001e

b. Effect tests
Source	parmd	DF	Sum of squares	F ratio	Prob > F
Genotype	3	3	62,456,335	106.1971	<0.0001e
Age (days)	1	1	4445	0.0227	0.8806
Age (days)∗Genotype	3	3	6,823,422	11.6021	<0.0001e

c. Post hoc Holm’s test for multiple comparisons
Group 1	Group 2	Rank	TTest p-value	Holm’s p-value	Significant
D35, LacZ	D35, WT	1	0.0001	0.00172	Y
D35, LacZ	D35, N174S	5	0.0001	0.002	Y
D35, LacZ	D35, Y225A	6	0.0001	0.00208	Y
D1, LacZ	D1, WT	8	0.0001	0.00227	Y
D1, LacZ	D1, N174S	9	0.0001	0.00238	Y
D35, Y225A	D35, WT	11	0.0001	0.00263	Y
D35, N174S	D35, WT	12	0.0001	0.00277	Y
D1, LacZ	D1, Y225A	15	0.0001	0.00333	Y
D1, WT	D35, WT	17	0.0001	0.00384	Y
D1, Y225A	D1, WT	18	0.0002	0.00416	Y
D35, LacZ	D1, LacZ	21	0.0045	0.00555	Y
D1, Y225A	D1, N174S	22	0.0522	0.00625	N
D1, N174S	D1, WT	23	0.0724	0.00714	N
D35, N174S	D1, N174S	25	0.1745	0.01	N
D1, Y225A	D35, Y225A	27	0.6978	0.01666	N
D35, Y225A	D35, N174S	28	0.9539	0.025	N

Abbreviation: DF, degrees of freedom.

a, 2-way ANOVA shows significant differences. b, Effect tests by genotype, age, and genotype-by-age. c, Post hoc Holm’s test for multiple pairwise comparisons. Only relevant pairwise interactions are shown, but the rank order is preserved from all comparisons.

^dparm: number of parameters.

^eSignificant differences.