Table 4.

Summary of statistical analysis of the area of the Kenyon cell clusters

a. Analysis of variance
Source	DF	Sum of squares	Mean square	F ratio	Prob > F
Model	7	575,614,234	82,230,605	19.6491
Error	88	368,276,203	4184956.9
C. Total	95	943,890,438			<0.0001d

b. Effect tests
Source	parme	DF	Sum of squares	F ratio	Prob > F
Genotype	3	3	481,137,511	38.3228	<0.0001d
Age (Days)	1	1	2,815,517	0.6728	0.4143d
Age (Days)∗Genotype	3	3	39,849,191	3.1740	0.0281d

c. Post hoc Holm’s test for multiple comparisons
Subject 1	Subject 2	Rank	TTest p-value	Holm p-value	Significant
D1, N174S	D1, Y225A	1	0.0001	0.00172	Y
D35, WT	D35, Y225A	4	0.0001	0.00192	Y
D1, N174S	D1, LacZ	5	0.0001	0.002	Y
D35, N174S	D35, Y225A	8	0.0001	0.00227	Y
D1, WT	D1, Y225A	9	0.0001	0.00238	Y
D35, WT	D35, LacZ	12	0.0002	0.00277	Y
D1, WT	D1, LacZ	14	0.0047	0.00312	N
D1, N174S	D1, WT	16	0.0142	0.00357	N
D35, WT	D1, WT	17	0.0293	0.00384	N
D35, N174S	D35, LacZ	18	0.0393	0.00416	N
D35, LacZ	D35, Y225A	19	0.0408	0.00454	N
D1, N174S	D35, N174S	20	0.0462	0.005	N
D35, WT	D35, N174S	22	0.0913	0.00625	N
D1, LacZ	D1, Y225A	23	0.1918	0.00714	N
D35, LacZ	D1, LacZ	24	0.3092	0.00833	N
D35, Y225A	D1, Y225A	27	0.6574	0.01666	N

a, 2-way ANOVA shows significant differences. b, Effect tests by genotype, age, and genotype-by-age. c, Post hoc Holm’s test for multiple pairwise comparisons. Only relevant pairwise interactions are shown, but the rank order is preserved from all comparisons.

^dSignificant differences.

^eparm: number of parameters.