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. 2023 Jun 6;27(14):2032–2044. doi: 10.1111/jcmm.17799

FIGURE 4.

FIGURE 4

The knockdown of TS suppressed cell survival, hampered DNA replication, inhibited cell migration, and induced cell apoptosis in gefitinib‐resistant cells. (A), the TS mRNA level of resistant cells with knockdown of TS. (B), the protein expression of TS in resistant cells with TS knockdown for 48 h. (C), the IC50 values of resistant cells with TS knockdown were measured by CCK8 assay under the treatment of gefitinib (GEF) for 48 h. (D, E), the colony formation ability of resistant cells after TS knockdown. (F, G), EdU staining assays used to detect DNA replication of resistant cells with TS knockdown in treatment of GEF for 48 h. (H, I), wound healing experiments were performed to evaluate the migrating ability of resistant cells with TS knockdown. (J, K), transwell assays were used to detect the number of cell migration after knockdown of TS. (L, M), flow cytometric analysis of cell apoptosis in resistant cells transfected with TS‐knockout plasmid and in treatment of GEF for 48 h. Three independent experiments were conducted. *p < 0.05, **p < 0.01.