FIGURE 7.

Gefitinib (GEF) and pemetrexed (PEM) combination treatment synergistically induced cellular senescence and hampered cell survival in gefitinib‐resistant cells. (A, B), EdU staining assays illustrated DNA proliferation in resistant cells treated with GEF and/or PEM for 48 h. The number of proliferating cells was counted with ImageJ and is displayed as the percentage of proliferating cells. (C, D), the migration of resistant cells treated with GEF and/or PEM for 48 h was evaluated with wound healing assays. The migration rate = healing area of scratch/initial area of scratch × 100%. (E, F), transwell assays of resistant cells under the above treatments. (G, H), SA‐β‐Gal staining assays in resistant cells treated with GEF and/or PEM. Histogram analysis showing the results from three independent experiments. **, p < 0.01.