表 1.

qRT-PCR对差异表达mRNA的引物序列

Primer sequence for qRT-PCR of differentially expressed mRNAs

Gene name	Forward primer (5'-3')	Reverse primer (5'-3')
Rat
β-actin	CTGGCTCCTAGCACCATGAAG	GAGCCACCAATCCACACAGA
TNF-α	CCCAGAAAAGCAAGCAACCA	CCTCGGGCCAGTGTATGAGA
IL-6	TCCTACCCCAACTTCCAATGCTC	TTGGATGGTCTTGGTCCTTAGCC
IL-1β	CACCTCTCAAGCAGAGCACAG	GGGTTCCATGGTGAAGTCAAC
Mouse
β-actin	GTGACGTTGACATCCGTAAAGA	GCCGGACTCATCGTACTCC
TNF-α	CAGGCGGTGCCTATGTCTC	CGATCACCCCGAAGTTCAGTAG
IL-6	TCTATACCACTTCACAAGTCGGA	GAATTGCCATTGCACAACTCTTT
IL-1β	GAAATGCCACCTTTTGACAGTG	TGGATGCTCTCATCAGGACAG