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Expression of HIF-1α and ability of chelating iron ions. (A) Iron ion chelation of GelMA, GelMA/3%PMAA scaffolds in FeCl3 solution. (B) Schematic of effect of scaffolds on BMSCs. (C, D) Protein expression of FTH and HIF-1α in BMSCs using Western blot. (E, F) The gene expression of FTH and HIF-1α in BMSC using quantitative real-time PCR. Data were analyzed via one-way ANOVA and are shown as mean ± standard deviation (*p < 0.05, **p < 0.01, ***p < 0.001, n = 3).
