Fig. 5.

All strains require glycogen for growth except L. gasseri 63 AM, which uses glucose at pH 5.0. Strains were grown for 15 d in standard MSVF, which contains 10 g/L of glycogen and 10 g/L of glucose, MSVF with half the level of glycogen (MSVF_5Gly) at (a) pH 4.0, (b) pH 4.5, and (c) pH 5.0, and MSVF with no glycogen (MSVF_0Gly) at (d) pH 4.0, (e) pH 4.5, and (f) pH 5.0 Samples were taken at 5-d intervals and the CFU/mL were determined. Each symbol represents the mean of three independent experiments ± SOM. Two-way ANOVA with Tukey’s multiple comparisons posttest was done to determine significant differences between time points across the growth curves. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001