Fig. 6.

Anakinra (ank) ameliorates autoimmune encephalitis in patient PBMC-humanized mice. A Schematic diagram of the experimental design. B Western blot of Claudin-1, Occludin, ZO-1, and Claudin-5 on cell lysates from hCMEC/D3 cells (n = 5/group) and statistical analysis of relative protein expression. C Relative changes in transendothelial electrical resistance (TEER) of in vitro BBB model (n = 5/group). D FITC–dextran recovery of in vitro BBB (n = 5/group). E Representative movement traces of patient-group mice (n = 6) and ank-treated patient + ank group mice (n = 6) in the open-field test. F Representative movement traces of mice from different groups (n = 6) in the elevated plus maze (EPM). G Immobility time of mice from different groups (n = 6) in the forced swimming test. H Western blot of tight junction proteins (Claudin-1, Occludin, ZO-1, and Claudin-5) on total tissue lysates from whole brains (n = 3/group). Western blot of hippocampal GluN1 (n = 3/group). I Statistical analysis of relative protein expression in H. J Statistical analysis of relative protein expression in H. *p < 0.05, **p < 0.01, Student’s t test