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. 2023 Jul 12;21:464. doi: 10.1186/s12967-023-04268-3

Fig. 2.

Fig. 2

CHCHD4 is downregulated in PASMCs and located in mitochondria. A Representative immunoblotting of CHCHD4 in isolated cells (n = 4), Normalized to β-Actin. B qPCR analysis of Chchd4 transcription in various cell types (n = 4). C Representative immunofluorescence of CHCHD4 and Mito tracker green in PASMCs. D Representative immunoblotting of CHCHD4 in cell components, Normalized to citrate synthase (CS) in mitochondria, PCNA in nucleus and β-Actin in cytoplasm. Data are shown as the mean ± SEM. P value is showed in each figure