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. 2023 Jul 12;21:464. doi: 10.1186/s12967-023-04268-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — CHCHD4 modulates mitochondrial dynamics by binding to SAM50. A Hierarchical cluster heatmap of genes downregulated in hypoxia compared with Normoxia and upregulated with AAV-CHCHD4 injection in hypoxia. Data were collected for genes of interest filtered by a fold change > 1.2 and p < 0.05. We identified 35 proteins satisfying our criteria. B Pathway enrichment analysis of the 35 genes of interest. C Representative immunoblotting of Opa1, Drp1 and Mfn1 in lung PA tissues from indicated groups (n = 6). Normalized to β-Actin. D Representative immunoblotting of Opa1, Drp1 and Mfn1 in isolated PASMCs from indicated groups (n = 6). Normalized to β-Actin. E PASMCs lysates were immunoprecipitated with anti-CHCHD4 or SAM50 antibodies and probed with anti-SAM50 or anti-CHCHD4 antibodies (n = 3 independent experiments). F Flag-agarose beads or HA agarose were incubated with lysates of HEK 293 cells transfected with either Flag-tagged SAM50 or together with HA-tagged CHCHD4, and immunoprecipitation was probed with anti-HA or Flag antibody (n = 3 independent experiments). G Immunofluorescence images present SAM50 (green) and CHCHD4 (red) colocalization (merge, yellow) in PASMCs (n = 3 biological replicates)