Fig. 7.

SAM50 knockdown abolishes the protective effects of CHHCD4 during hypoxia. A, B Representative confocal images of Mitochondrial Tracker in indicated group and quantification of the mitochondrial mean length (n = 73–108 PASMCs). White arow indicated the mitochondrial length. C Representative immunoblotting of SAM50, Opa1, Drp1 and Mfn1 in isolated PASMCs from indicated groups (n = 6). Normalized to β-Actin. D, E Representative images and summary data of the EdU incorporation assay. EdU positivity indicated that the cells were proliferating (n = 3 biological replicates). F, G Representative images and summary data of the Transwell assay, which indicated the migration ability of PASMCs in indicated groups (n = 3 independent experiments). Data are shown as the mean ± SEM. P value is showed in each figure