Table 1.
Primer sequences for RT-qPCR
| Gene | Forward primer sequence (5’-3’) | Reverse primer sequence (5’-3’) |
|---|---|---|
| GAPDH1 | CTGGAGAAACCTGCCAAGTATG | GGTGGAAGAATGGGAGTTGCT |
| Alpl | CGGCACCTGCCTTACCAACT | ACTGTGGAGACGCCCATACC |
| Spp1 | GATGAACAGTATCCCGATGCCA | GTCTTCCCGTTGCTGTCCTGA |
| Runx2 | CAGTATGAGAGTAGGTGTCCCGC | AAGAGGGGTAAGACTGGTCATAGG |
| Col1a1 | CGTGGAAACCTGATGTATGCTTG | CCTATGACTTCTGCGTCTGGTGA |
| SP7 | CTGGGAAAAGGAGGCACAAAGA | GGGGAAAGGGTGGGTAGTCATT |
| Bglap | AGGGCAGTAAGGTGGTGAATAGA | GAAGCCAATGTGGTCCGCTA |
| GAPDH2 | CCTCGTCCCGTAGACAAAATG | TGAGGTCAATGAAGGGGTCGT |
| C-Fos | CGAAGGAACGGAATAAGATG | TGGGAAGCCAAGGTCATCG |
| Acp5 | CACGATGCCAGCGACAAGA | TGAAGCGCAAACGGTAGTAAGG |
| CTSK | GTTGACTTCCGCAATCCTTACC | CAGCAGAAACTTGAACACCCAC |
| Nfatc1 | CACTCCACCCACTTCTGACTTCC | GGCTGCCTTCCGTCTCATAGTG |
| Calcr | CAACCGAACCTGGTCCAACTAT | GACAAGGAGTGACCCACAAGAG |
| MMP9 | GCTGGCAGAGGCATACTTGTAC | CAGTATGTGATGTTATGATGGTCCC |
Note: GAPDH1 represents glyceraldehyde-3-phosphate dehydrogenase gene of rat, and GAPDH2 represents glyceraldehyde-3-phosphate dehydrogenase gene of mouse.