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. Author manuscript; available in PMC: 2023 Jul 13.
Published in final edited form as: Dev Biol. 2022 Sep 5;491:56–65. doi: 10.1016/j.ydbio.2022.08.005

Fig. 7. Irf4 knockdown produces excess pigment cells.

Fig. 7.

A. A double in situ showing pigment cells expressing pks1 and blastocoelar cells expressing irf4 at the tip of the archenteron at late gastrula stage. B. In situ of Pigment cells at late gastrula stage in control embryos. C. In situ of pks1-expressing pigment cells at late gastrula stage in irf4-morpholino knockdown embryos. D. An irf4 knockdown larval arm contains an excess number of pigment cells. Insert in B shows a UMAP from a temporal scRNA-seq analysis (Massri et al., 2021). The strongest expression of Pks1 appears in the lineage leading to pigment cells (P). The branch leading to (B) is the blastocoelar lineage. Inserts within E, F and G, H show that Astacin4 and Ron are genes expressed exclusively in the Blastocoelar lineage. Knockdowns of irf4 reduces or eliminates expression of astacin4 and ron (F and H). UMAP inserts show the localized expression of these two genes in the blastocoelar lineage. Bar = 25 μm.