Figure 3:

Plasma ACTH and corticosterone response to 30 min of restraint in male and female rats Before and then During abstinence from opioids in control rats and rats under sleep restricted conditions (N values as in Figure 2; mean/SE). All statistical symbols are P < 0.05. Symbol colors are specific to AC (blue) or SR (red) rats. a, different from 0 min; b above the dashed line indicates an overall difference between AC and SR “During” by Hotelling’s test (P<0.019); c, different from “Before” at that time point. c above the dashed line indicates an overall difference from “Before” by Hotelling’s test for AC (P=0.0027) and SR (P=0.026) rats. d, different from male within same treatment at same time point. For overall ANOVA and all specific comparisons, see link to Supplementary Table 2 in the reference list (2).

Figure 3 Alt Text: The corticosterone response to restraint was attenuated in sleep restricted male rats during abstinence compared to before abstinence (bottom panels). Opioid abstinence attenuated the ACTH response to restraint in female rats (top right panel). Sleep restricted female rats did not achieve the full restraint recovery nadir in corticosterone (bottom right panel).