Table 2. Results of the Cq values of the tested extraction kits show differences in target extraction efficiency of MNV RNA whether ethanol (EtOH) or isopropanol (IPA) was used during the extraction.
| N(MNV) | Cq | |||||||
|---|---|---|---|---|---|---|---|---|
| Kit 1 | Kit 2 | Kit 3 | Kit 4 | |||||
| EtOH (O) | IPA (M) | EtOH (O) | IPA (M) | EtOH (M) | IPA (O) | EtOH (O) | IPA (M) | |
| undiluted | 26.30 | 29.99 | 33.04 | 30.29 | 31.72 | 28.94 | 37.83 | 38.57 |
| 10-1 | 29.02 | 33.53 | 35.81 | 32.92 | 33.96 | 31.48 | 40.44 | 41.50 |
| 10-2 | 32.49 | 37.03 | 39.96 | 36.59 | 37.49 | 34.51 | NA | NA |
| 10-3 | 36.60 | 40.35 | 43.29 | 39.96 | 40.79 | 39.33 | NA | NA |
MNV=murine norovirus standard solution (various dilutions), O=original protocol, M=modified protocol, Cq=number of cycles at which the target analyte (after the RT-PCR reaction) curve intersects the threshold line