Figure 1.
The concept of using single EV SERS approach in liquid biopsy of GBM patients. (a) Possibilities of using MoSERS microchip embedded microfluidic device for liquid biopsy of blood EVs for early detection and monitoring of cancer (i): A microfluidic prototype embedded with MoSERS microchips with the size of 0.1 cm × 0.1 cm for sample delivery and detection (ii). Single EV SERS spectrum analyzed by machine learning algorithm (CNN) to stratify molecular alterations (iii). (b) The nanosurface microfluidic embedded with MoSERS microchip perform for SERS identification of single EVs of Glioblastoma cancer. Single EVs are entrapped in the plasmonic nanocavities, with one EV per cavity, and SERS is performed utilizing high-field enhancement at the nanocavity edges. (c) Single EV entrapment in the MoS2 encapsulated nanocavities of MoSERS microchip. Schematic of MoSERS microchip fabrication and related SEM images of each step.