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. 2023 Jun 20;17(13):12101–12117. doi: 10.1021/acsnano.2c11884

Figure 2.

Figure 2

Multivalent IFs presenting pMHC activate and expand antigen-specific primary mouse T cells ex vivo. (A) Schematic overview of IF-pMHC(SIIN) and free pMHC(SIIN) for stimulation of murine OT-I T cells. (B) Flow cytometry quantification of the percentage of activated CD25+ OT-I T cells at different time points. Statistical significance was tested with two-way ANOVA on logit-transformed data. (C) The production of IFNγ by OT-I T cells at different time points was determined by ELISA. Statistical significance was tested with two-way ANOVA on log-transformed data. (D) Schematic overview of an experiment where IF-pMHC(SIIN) and free pMHC(SIIN) are washed off from OT-I T cells after 30 or 90 min incubation. (E,F) Representative example of the CTV dilution (E) and calculated division index of OT-I T cells (F) after three days of stimulation, either without removing the stimulation (no wash) or by washing away IF/free pMHC after 30 or 90 min, respectively. Statistical significance was determined by two-way ANOVA on log-transformed data with posthoc Sidak’s multiple comparison test. (B–C,F) n = 3 in three independent experiments. p-Values are indicated in the figure.