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. Author manuscript; available in PMC: 2023 Jul 13.
Published in final edited form as: Nat Protoc. 2022 Nov 16;18(2):396–423. doi: 10.1038/s41596-022-00766-8
Component Reaction volumea (total/per tube) (μl)
RDC product 8,960/1,792
20× AcTEV buffer 500/100
0.1 M DTT 100/20
AcTEV 46/9.2
Ultrapure water 394/78.8
Total 10,000/2,000
a
The reaction needs to be divided equally among five tubes.