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. Author manuscript; available in PMC: 2023 Jul 13.
Published in final edited form as: Nat Protoc. 2022 Nov 16;18(2):396–423. doi: 10.1038/s41596-022-00766-8
Component Stock concentration Reaction volume (μl)
Ultrapure water – 495
5× Q5 high-fidelity buffer 5× 200
10 mM dNTP mix 10 mM each 20
Forward & reverse DDP primer mix 5 μM each 75
Q5 high-fidelity DNA polymerase 2 U/μl 10
Diluted oligo pool 3.13 nM 200
Total – 1,000