| Temperature (°C) | Time | No. of cycles |
|---|---|---|
| 98 | 30 s | 1 |
| 98 | 10 s | 5 |
| 55 | 30 s | |
| 72 | 30 s | |
| 98 | 10 s | 10 |
| 72 | 30 s | |
| 72 | 5 min | 1 |
| 4 | ∞ | – |
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