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| Component | Stock concentration | Final concentration in reaction | Reaction volumea (total/per tube) (μl) |
|---|---|---|---|
| DDP product | >50 ng/μl | >15.75 ng/μl | 945/189 |
| AmpliScribe T7-Flash 10× reaction buffer | 10× | 1× | 300/60 |
| 25 mM NTP mix | 25 mM each (after combining 1:1:1:1) | 9 mM each | 1,080/216 |
| 100 mM DTT | 100 mM | 10 mM | 300/60 |
| RoboGuard RNase inhibitor | 40 U/μl | 1 U/μl | 75/15 |
| AmpliScribe T7-Flash RNA polymerase | 10× | 1× | 300/60 |
| Total | – | – | 3,000/600 |
