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. Author manuscript; available in PMC: 2023 Jul 13.
Published in final edited form as: Nat Protoc. 2022 Nov 16;18(2):396–423. doi: 10.1038/s41596-022-00766-8
Component Stock concentration Final concentration in reaction Reaction volume (μl)
Ultrapure water – – 1,430 – Y
10× T4 RNA ligase 2 buffer 10× 1× 200
MRP product Variable depending on MRP concentration (measured in Step 24) 50–100 μM Y
Puromycin adapter 1,000 μM 110 μM 220
RNase inhibitor, murine 40 U/μl 1 U/μl 50
T4 RNA ligase 2 10 U/μl 0.5 U/μl 100
Total – – 2,000