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. Author manuscript; available in PMC: 2023 Jul 13.
Published in final edited form as: Nat Protoc. 2022 Nov 16;18(2):396–423. doi: 10.1038/s41596-022-00766-8
Component Stock concentration Final concentration in reaction Reaction volumea (total/per tube) (μl)
PURExpress Component A 2.5× 1× 2,800/560
PURExpress Component B 3.3× 1× 2,100/420
RNase inhibitor, murine 40 U/μl 1.6 U/μl 280/56
PAL product Variable depending on PAL concentration (measured in Step 34) 2.0 μM Y
Ultrapure water – Variable 1,820 − Y
Total – – 7,000/1,400
a
The reaction needs to be divided equally among five tubes.