Component	Stock concentration	Final concentration in reaction	Reaction volumea (total/per tube) (μl)
PTC product	PTC product (collected in Step 72)	–	6,720/960
5× ProtoScript II reverse transcriptase reaction buffer	5×	1×	2,240/320
10 mM dNTP mix	10 mM each	500 μM each	560/80
0.1 M DTT	0.1 M	10 mM	1,120/160
RNase inhibitor, murine	40 U/μl	1 U/μl	280/40
ProtoScript II reverse transcriptase	200 U/μl	5 U/μl	280/40
Total	–	–	11,200/1,600

^a

The reaction needs to be divided equally among seven tubes.