Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Component | Stock concentration | Final concentration in reaction | Reaction volumea (total/per tube) (μl) |
|---|---|---|---|
| Reverse transcription product | Product from Step 76 | – | 11,200/1,600 |
| RNase cocktail | RNase A: 0.5 U/μl RNase T1: 20 U/μl |
RNase A: 0.043 U/μl RNase T1: 1.7 U/μl |
1,120/160 |
| RNase H | 5 U/μl | 0.32 U/μl | 840/120 |
| Total | – | – | 13,160/1,880 |
