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Protoplast cultures and secondary metabolite production. (A) Main plant and explant sources, (B) Isolation of plant cells from leaf tissue, (C) Cell wall removal and obtaining protoplasts, (D) Culturing the protoplasts in liquid media, (E) Obtaining callus, (F) Isolation of secondary metabolites from protoplast derived calli (Copyrighted illustration from Prof. Ozyigit).
