Table 2.
Primer pairs used for RT-qPCR analyses 1.
| Gene Name/Abbreviation | Accession Number | Primer Sequence (5′-3′) |
|---|---|---|
| β-actin | NM_205518 | F:GAGAAATTGTGCGTGACATCA |
| R:CCTGAACCTCTCATTGCCA | ||
| FATP4 | XM_015279553.3 | F:GAGCCGCATCCTCAACCTG |
| R:GCTGCCATTCCTGCCTTCC | ||
| PepT1 | NM_204365.2 | F:TCACTGTTGGCATGTTCCT |
| R:TTCGCATTGCTATCACCTA | ||
| CAT-1 | NM_001145490.1 | F:TACAACAGGTGAGGAGGTG |
| R:AAGCCACAAAGCAGATGAG | ||
| ZO-1 | XM_040680632.1 | F:GGCAAGTTGAAGATGGTGGT |
| R:ATGCCAGCGACTGAATTTCT | ||
| MUC2 | XM_040701667.1 | F:AACTCCTCCTTTGTATGCG |
| R:ATTCAACCTTCTGCCCTAA | ||
| OCLN | NM_205128.1 | F:CCTCATCGTCATCCTGCTCTG |
| R:GCCACGTTCTTCACCCACTC | ||
| IL-10 | NM_001004414.2 | F:TGTCACCGCTTCTTCACCT |
| R:TCCCGTTCTCATCCATCTT | ||
| IL-6 | NM_204628.1 | F: CTCCTCGCCAATCTGAAGTC |
| R:CCCTCACGGTCTTCTCCATA | ||
| IL-1 β | NM_204524.1 | F:GCTCTACATGTCGTGTGTGATGAG |
| R:TGTCGATGTCCCGCATGA | ||
| TNF-α | NM_204267.2 | F:TACCCTGTCCCACAACCTG |
| R:GGCGGTCATAGAACAGCAC |
1 F, forward; R, reverse; FATP4, fatty acid transport protein 4; PepT1, peptide transporter 1; CAT-1, cationic amino acid transporter 1; ZO-1, zonula occludens-1; MUC2, mucin 2; OCLN, Occludin; IL-10, interleukin-10; IL-6, interleukin-6; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α.