Table 1. Results from the main data analysis models.
(1) | (2) | (3) | (4) | (5) | (6) | (7) | (8) | (9) | (10) |
---|---|---|---|---|---|---|---|---|---|
Metric | Group | Pre/Post-Bout | BoutNum | Group * Pre/Post-Bout | Group * BoutNum | Pre/Post-Bout * BoutNum | Group * Pre/Post-Bout * BoutNum | Group (at 6 weeks recovery) | |
Weight | F3,123 = 5.95 | F1,290 = 4.46 | F5,294 = 2.42 | F2,291 = 2.69 | F15,292 = 1.72 | F5,291 = 1.65 | F10,291 = 2.06 | F2 = 4.06 | |
p = 8.04 × 10 −4 | p = 0.04 | p = 0.04 | p = 0.07 | p = 0.05 | p = 0.15 | p = 0.03 | p = 0.04 | ||
Glucose | Fasted | F3,299 = 0.97 | F1,296 = 3.54 | F5,299 = 4.10 | F2,295 = 0.63 | F5,296 = 5.68 | F5,296 = 5.68 | F10,296 = 3.13 | F2 = 0.78 |
p = 0.41 | p = 0.06 | p = 1.29 × 10 −3 | p = 0.53 | p = 0.01 | p = 5.04 × 10 −5 | p = 7.89 × 10 −4 | p = 0.48 | ||
Fed | F3,256 = 2.33 | F1,295 = 1.57 | F5,298 = 1.49 | F2,295 = 0.31 | F15,298 = 1.03 | F5,295 = 3.61 | F10,295 = 3.22 | F2 = 1.51 | |
p = 0.07 | p = 0.21 | p = 0.19 | p = 0.73 | p = 0.42 | p = 3.51 × 10 −3 | p = 5.86 × 10 −4 | p = 0.25 | ||
Fold-change | F3,326 = 4.17 | F1,300 = 6.86 | F5,298 = 1.21 | F2,297 = 0.98 | F15,298 = 1.24 | F5,297 = 1.72 | F10,296 = 1.18 | F2 = 1.05 | |
p = 6.45 × 10 −3 | p = 9.27 × 10 −3 | p = 0.30 | p = 0.38 | p = 0.24 | p = 0.13 | p = 0.30 | p = 0.37 | ||
Ketones | Fasted | F1,230 = 0.11 | F1,286 = 0.37 | F5,291 = 1.01 | F2,286 = 0.71 | F15,298 = 1.61 | F5,286 = 2.77 | F10,287 = 3.58 | F2 = 0.59 |
p = 0.96 | p = 0.54 | p = 0.41 | p = 0.49 | p = 0.07 | p = 0.02 | p = 1.71 × 10 −4 | p = 0.56 | ||
Fed | F3,284 = 3.60 | F1,291 = 8.27 | F5,296 = 9.52 | F2,291 = 2.26 | F15,295 = 2.67 | F5,291 = 4.55 | F10,291 = 2.58 | F2 = 0.94 | |
p = 0.01 | p = 4.32 × 10 −3 | p =1.97 × 10 −8 | p = 0.10 | p = 7.68 × 10 −4 | p = 5.18 × 10 −4 | p = 5.21 × 10 −3 | p = 0.41 | ||
Fold-change | F2,292 = 1.86 | F1,283 = 5.56 | F5,289 = 5.48 | F2,283 = 2.41 | F12,287 = 2.29 | F5,283 = 6.80 | F10,284 = 4.47 | F2 = 1.46 | |
p = 0.14 | p = 0.02 | p = 7.75 × 10 −5 | p = 0.09 | p = 4.48 × 10 −3 | p = 5.23 × 10 −6 | p = 7.26 × 10 −6 | p = 0.26 | ||
Uric Acid | Fasted | F3,269 = 3.76 | F1,296 = 0.49 | F5,300 = 2.19 | F2,296 = 0.47 | F15,299 = 2.25 | F5,297 = 2.25 | F10,297 = 2.59 | F2 = 0.48 |
p = 0.01 | p = 0.48 | p = 0.06 | p = 0.62 | p = 0.07 | p = 0.05 | p = 5.00 × 10 −3 | p = 0.62 | ||
Fed | F3,187 = 1.49 | F1,275 = 3.12 | F5,298 = 1.89 | F2,295 = 3.71 | F15,297 = 1.30 | F5,295 = 1.47 | F10,295 = 1.49 | F2 = 0.60 | |
p = 0.22 | p = 0.08 | p = 0.10 | p = 0.03 | p = 0.20 | p = 0.20 | p = 0.14 | p = 0.56 | ||
Fold-change | F3,329 = 3.51 | F1,297 = 1.89 | F5,301 = 3.96 | F2,298 = 1.31 | F15,301 = 1.49 | F5,298 = 0.80 | F10,298 = 1.86 | F2 = 0.68 | |
p = 0.02 | p = 0.17 | p = 1.70 × 10 −3 | p = 0.27 | p = 0.11 | p = 0.55 | p = 0.05 | p = 0.52 |
Note:
Columns 3–9 show the results of the linear mixed-effect model, “metric~group * pre/post-bout * bout number”, with bird identity as a random effect. The metrics are listed in columns 1–2, group is treatment group (captivity-only, low stress, medium stress, and high stress), pre/post-bout refers to when the sample was taken (pre-bout or post-bout), and bout num is the bout number (1–6 from Fig. 1). Column 10 reflects the results of the ANOVA on the samples taken 6 weeks after the experiment ended, where the model is “metric~group”. Cells that are bolded contain p-values less than 0.05.