. 2023 Jul 10;11:e15661. doi: 10.7717/peerj.15661

Table 4. Results from data analyses on samples taken at the end of each bout (post-bout).

(1)	(2)	(3)	(4)	(5)	(6)	(7)	(8)
Metric		Group	BoutNum	Group * BoutNum	Low stress	Medium stress	High stress
Weight		F_2,73 = 2.20	F_5,110 = 4.83	F_10,111 = 2.97	F_5,39 = 4.67	F_5,31 = 1.76	F_5,42 = 1.14
		p = 0.12	p = 4.93 × 10 ⁻⁴	p = 2.39 × 10 ⁻³	p = 1.98 × 10 ⁻³	p = 0.15	p = 0.35
Glucose	Fasted	F_2,136 = 0.91	F_5,115 = 2.29	F_10,116 = 2.51	F_5,40 = 3.55	F_5,34 = 0.85	F_5,42 = 1.99
	Fasted	p = 0.41	p = 0.05	p = 9.05 × 10 ⁻³	p = 9.44 × 10 ⁻³	p = 0.52	p = 0.02
	Fed	F_2,124 = 0.94	F_5,113 = 3.33	F_10,114 = 3.30	F_5,40 = 3.56	F_5,33 = 1.15	F_5,42 = 3.33
	Fed	p = 0.39	p = 7.61 × 10 ⁻³	p = 8.69 × 10 ⁻⁴	p = 9.36 × 10 ⁻³	p = 0.35	p = 0.01
	Fold-change	F_2,137 = 1.79	F_5,113 = 3.16	F_10,116 = 1.30
	Fold-change	p = 0.17	p = 0.01	p = 0.24
Ketones	Fasted	F_2,130 = 0.66	F_5,111 = 1.07	F_10,112 = 3.94	F_5,38 = 1.22	F_5,32 = 6.12	F_5,41 = 2.35
	Fasted	p = 0.52	p = 0.38	p = 1.28 × 10 ⁻⁴	p = 0.32	p = 4.50 × 10 ⁻⁴	p = 0.06
	Fed	F_2,126 = 0.59	F_5,113 = 1.63	F_10,114 = 4.09	F_5,39 = 1.64	F_5,33 = 3.31	F_5,42 = 7.70
	Fed	p = 0.56	p = 0.16	p = 7.91 × 10 ⁻⁵	p = 0.17	p = 0.02	p = 3.27 × 10 ⁻⁵
	Fold-change	F_2,132 = 0.79	F_5,110 = 1.00	F_10,112 = 5.36	F_5,38 = 0.86	F_5,32 = 5.62	F_5,41 = 8.46
	Fold-change	p = 0.46	p = 0.42	p = 1.91 × 10 ⁻⁶	p = 0.51	p = 8.07 × 10 ⁻⁴	p = 1.41 × 10 ⁻⁵
Uric acid	Fasted	F_2,105 = 1.47	F_5,114 = 1.81	F_10,115 = 3.45	F_5,40 = 1.54	F_5,33 = 5.05	F_5,42 = 3.40
	Fasted	p = 0.23	p = 0.11	p = 5.43 × 10 ⁻⁴	p = 0.20	p = 1.51 × 10 ⁻³	p = 0.01
	Fed	F_2,115 = 1.47	F_5,113 = 1.63	F_10,115 = 2.05	F_5,40 = 1.39	F_5,32 = 4.31	F_5,42 = 2.63
	Fed	p = 0.23	p = 0.16	p = 0.03	p = 0.25	p = 4.02 × 10	p = 0.04
	Fold-change

Note:

Columns 3–5 show the results of the linear mixed-effect model, “metric~group * bout num”, with bird identity as a random effect. Metrics are listed in columns 1–2, group is treatment group (captivity-only, low stress, medium stress, high stress), and bout num is the bout number (1–6). There are no results for fold-change uric acid because there was no significant effect of group or significant interaction with group in the main analysis (shown in Table 1). For the model, “metric~group * bout num”, if there was a significant effect of group or a significant interaction with group, the groups were split and four separate models (one for each group) was run, in which the model was “metric~bout num”, with bird identity as a random effect. The results from these four models are shown in columns 6–8. There are no results in columns 6–9 for fold-change glucose because the previous model (“metric~group * bout num”) did not show a significant effect of group or a significant interaction with group. There is no column for the captivity-only group because they were only sampled at pre-bout timepoints to reduce disturbance. Cells that are bolded contain p-values less than 0.05.