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. 2023 Jun 26;57(27):9925–9933. doi: 10.1021/acs.est.3c01134

Table 1. Concentrations of PS Particle Dispersions and Residual Styrene Concentration at Varying PS Contents in Cell Culturea.

nomenclature PS (μg/mL) styrene (%) PS (%) residual styrene at varying PS (μg/mL)
300 500 1000 1900
MIP 27,400 0.875 99.13 2.65 4.41 8.82 16.77
MIPA 30,500 0.214 99.79b 0.64 1.07 2.14 4.07
MHP 17,300 0.030 99.97 0.09 0.15 0.30 0.56
MHPA 21,030 0.038 99.96b 0.11 0.19 0.38 0.71
S0     86.7        
SIP 19,360 0.572 99.43 1.73 2.88 5.75 10.93
SIPA 20,780 0.235 99.77b 0.71 1.18 2.35 4.47
SHP 15,510 0.028 99.97 0.08 0.14 0.28 0.53
SHPA 17,260 0.043 99.96b 0.13 0.22 0.43 0.83
a

Calculated final styrene concentration for PS particle dispersions at PS concentrations of 300, 500, 1000, and 1900 μg/mL.

b

The purity of the autoclaved dispersions changed. This can be ascribed to the overall outcome of the evaporation and condensation of water and styrene (boiling point 145 °C, density 0.91 g/mL) in the glass vial during the autoclaving process (121 °C). Purity refers to the percentage of the PS mass accounting for the total mass of PS and styrene in the dispersions. MIP, standard PS model particle dispersion as received; MIPA, autoclaved MIP; MHP, rapidly dialyzed MIP against methanol/water (50/50) mixed solvents; MHPA, autoclaved MHP; S0, in-house synthesized PS particle dispersion without purification; SIP, S0 dialyzed against water for 40 days; SIPA, autoclaved SIP; SHP, rapidly dialyzed SIP against methanol/water (50/50) mixed solvents; SHPA, autoclaved SHP.