Table 1. Concentrations of PS Particle Dispersions and Residual Styrene Concentration at Varying PS Contents in Cell Culturea.
nomenclature | PS (μg/mL) | styrene (%) | PS (%) | residual
styrene at varying PS (μg/mL) |
|||
---|---|---|---|---|---|---|---|
300 | 500 | 1000 | 1900 | ||||
MIP | 27,400 | 0.875 | 99.13 | 2.65 | 4.41 | 8.82 | 16.77 |
MIPA | 30,500 | 0.214 | 99.79b | 0.64 | 1.07 | 2.14 | 4.07 |
MHP | 17,300 | 0.030 | 99.97 | 0.09 | 0.15 | 0.30 | 0.56 |
MHPA | 21,030 | 0.038 | 99.96b | 0.11 | 0.19 | 0.38 | 0.71 |
S0 | 86.7 | ||||||
SIP | 19,360 | 0.572 | 99.43 | 1.73 | 2.88 | 5.75 | 10.93 |
SIPA | 20,780 | 0.235 | 99.77b | 0.71 | 1.18 | 2.35 | 4.47 |
SHP | 15,510 | 0.028 | 99.97 | 0.08 | 0.14 | 0.28 | 0.53 |
SHPA | 17,260 | 0.043 | 99.96b | 0.13 | 0.22 | 0.43 | 0.83 |
Calculated final styrene concentration for PS particle dispersions at PS concentrations of 300, 500, 1000, and 1900 μg/mL.
The purity of the autoclaved dispersions changed. This can be ascribed to the overall outcome of the evaporation and condensation of water and styrene (boiling point 145 °C, density 0.91 g/mL) in the glass vial during the autoclaving process (121 °C). Purity refers to the percentage of the PS mass accounting for the total mass of PS and styrene in the dispersions. MIP, standard PS model particle dispersion as received; MIPA, autoclaved MIP; MHP, rapidly dialyzed MIP against methanol/water (50/50) mixed solvents; MHPA, autoclaved MHP; S0, in-house synthesized PS particle dispersion without purification; SIP, S0 dialyzed against water for 40 days; SIPA, autoclaved SIP; SHP, rapidly dialyzed SIP against methanol/water (50/50) mixed solvents; SHPA, autoclaved SHP.