Figure 4.

Tumor inhibition by OMVs_Δ60 ISV in a two-tumor mouse model. (A) BALB/c mice were injected s.c. with 2.5 × 10⁵ CT26 cells in both flanks, and the first of the two tumors to reach a palpable size of 50–100 mm³ was intratumorally vaccinated three times, two days apart. Mice received either PBS, OMVs_Δ60, or OMVs_Δ60 + pentatope (a mixture of five mutation-derived, tumor-specific T cell neo-epitopes). (B) Tumor growth was monitored on both sides over a period of 26 days. The figure shows data from two (OMVs_Δ60, or OMVs_Δ60 + pentatope) or three (PBS control) independent experiments using groups of five mice each. Statistical significance was assessed using two-tailed Student’s t test. Error bars: mean ± SEM. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.