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. 2023 Jul 7;12(13):1804. doi: 10.3390/cells12131804

Figure 1.

Figure 1

Effects of APAP, IB, GEN, and MEHP on TM4 and PND8 Sertoli cell viability measured by MTT assays. (A) TM4 cell viability after 24 h treatments with vehicle (Veh), APAP (A), IB, GEN (G) or MEHP (M) at 10, 50, and 100 µM, alone or mixed, in 10% Reg-FBS. (B) TM4 cell growth at 24 and 48 h in Reg-FBS (circles) vs. CS-FBS (diamonds) (raw absorbance values). (C) TM4 cell viability in medium containing 10% Reg-FBS (closed symbols) or CS-FBS (opened symbols) up to 72 h. Black: vehicle. Colors indicate treatments. (D) PND8 Sertoli cell viability and proliferation assessed by MTT assay after 24 h and 48 h treatments with vehicle (Veh), APAP (A), GEN (G), or their mixtures (AG) at 10, 50, and 100 µM, in 10% CS-FBS. Data are means ± SEM of three independent experiments (TM4 cells) or two experiments (PND8 Sertoli cells) performed in triplicates. A one-way ANOVA, * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001.