Table 1.
Summary of the substrate efflux activities of TMH4 and TMH10 mutants.
| Substrates | TMH4,10-14A | TMH4,10-11A | TMH4-7A | TMH10-7A |
|---|---|---|---|---|
| BD-CsA | 33 ± 3 | 3 ± 0.6 | 89 ± 3 | 85 ± 2 |
| BD-EDA | 22 ± 5 | 66 ± 6 | 93 ± 67 | 46 ± 2 |
| BD-Prazosin | 2 ± 0.6 | 54 ± 4 | 40 ± 10 | 18 ± 13 |
| BD-Verapamil | ND | 3 ± 0.6 | 26 ± 12 | 3 ± 2 |
| Calcein-AM | 23 ± 9 | 58 ± 9 | 94 ± 4 | 97 ± 3 |
| Daunorubicin | 21 ± 1 | 34 ± 2 | 64 ± 6 | 54 ± 9 |
| Flutax-1 | ND | 4 ± 2 | 98 ± 4 | 92 ± 7 |
| LDS 751 | 31 ± 8 | 43 ± 6 | 89 ± 8 | 91 ± 8 |
| NBD-CsA | 3 ± 1 | 4 ± 1 | 95 ± 9 | 94 ± 11 |
| R6 | 13 ± 11 | 6 ± 2 | 54 ± 6 | 13 ± 1 |
| R6G | 43 ± 3 | 78 ± 3 | 90 ± 1 | 95 ± 1 |
| Rh123 | 3 ± 0.58 | 28 ± 5 | 93 ± 3 | 90 ± 3 |
| Rhod-2 AM | ND | 5 ± 2 | 96 ± 6 | 96 ± 5 |
| TMRE | 26 ± 5 | 85 ± 1 | 100 ± 0 | 98 ± 3 |
| X-Rhod-1 AM | 4 ± 1 | 4 ± 1 | 96 ± 6 | 99 ± 1 |