Impaired locomotor recovery following contusion SCI in AQP4−/− mice. AQP4−/− mice had a worse functional outcome as revealed by BMS (A) and BMS subscores (B) compared with WT mice (white circles, WT; black circles, AQP4−/−). Data are represented as mean ± SEM, n = 10 each group. Statistical comparisons were made using repeated-measures ANOVA with Bonferroni post-hoc test, * p < 0.05, ** p < 0.01. (C) Representative images of footprint analysis (upper left, WT sham; lower left, WT 42 days post-injury, i.e., dpi; upper right, AQP4−/− sham; lower right, AQP4−/− 42 dpi). AQP4−/− mice showed significantly decreased stride length in both forelimbs (D) and hindlimbs (E), decreased width of hindlimb footprints (F), and increased toe drag ratio (G) (white bars, WT; black bars, AQP4−/−). Data are represented as mean ± SEM, n = 10 each group. Statistical significance was evaluated using a Student’s t-test or Mann–Whitney U test, * p < 0.05, ** p < 0.01. Figure and figure legend adopted from [90] with permission.