Figure 2.

Effect of volume-regulated anion channel (VRAC) blockers, phloretin or DCPIB, on HOC-modulated electrical activity of VP neurons in acute SON slices. (A). Changes in the HOC-mediated time-dependent firing rate of VP neurons in the presence of 30 μmol/L phloretin in whole−cell patch-clamp recordings: (a) representative episodes of VP neuronal firing activity; (b) heatmap of the relative firing rate; and (c) bar graphs of average VP neuronal firing rates and data from individual neurons shown by line-connected points (n = 11; *, p < 0.05; **, p < 0.01 compared with 0 min HOC; ANOVA, Bonferroni. (B). Changes in the HOC-modulated time-dependent firing activity of VP neurons in the presence of 20 μmol/L DCPIB: (a–c), annotation as in (A); ((c), n = 5; **, p < 0.01, compared with 0 min HOC; ANOVA, Sidak). For other annotations and abbreviations, refer to Figure 1.