Figure 6.

Effects of phloretin on HOC-evoked astrocytic VRAC currents. (A). HOC-evoked VRAC currents in astrocytes at late HOC stages (a) are dampened by subsequent application of 30 μmol/L of phloretin. (b) Relative current−voltage curves obtained from astrocytes under different conditions normalized to currents recorded just prior to the HOC onset (I_{HOC 0 min}; n = 7; *, p < 0.05, compared with 0 min HOC; #, p < 0.05, compared with 10 min HOC; ANOVA, Bonferroni). (c) A series of voltage steps, initiated from a holding potential of −60 mV, were used to test for VRAC currents in astrocytes. (d) Schematics of liquid perfusion protocol. (B). Pre-application of 30 μmol/L phloretin occludes the development of HOC-evoked VRAC currents in astrocytes (a). (b) Relative current-voltage curves obtained from astrocytes under different conditions, normalized to current at control conditions (I_CTR; n = 6; ANOVA, Bonferroni). (c) Schematics of liquid perfusion protocol. Other annotations as in Figure 1.