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. 2023 Jun 26;12(13):1723. doi: 10.3390/cells12131723

Figure 7.

Figure 7

Effects of TGN-020 on HOC-evoked VRAC currents in cultured hypothalamic astrocytes. (A). (a) HOC-evoked VRAC currents in astrocytes, after developing HOC for 10 min, are not affected by subsequent application of 10 μmol/L TGN-020; (b) Current-voltage curves of outwardly rectifying VRAC currents obtained from astrocytes under different conditions, normalized to currents recorded just prior to the HOC onset (I HOC 0 min; n = 12; *, p < 0.05, compared with 0 min HOC; #, p < 0.05, compared with 10 min HOC; ANOVA, Bonferroni). (c) A series of voltage steps, initiated from a holding potential of −60 mV, were used to test for VRAC currents in astrocytes. (d) Schematics of liquid perfusion protocol. (B). (a) Pre-application of 10 μmol/L of TGN-020 occluded development of HOC-evoked VRAC currents in astrocytes. (b) Current-voltage curves obtained from astrocytes under different conditions, normalized to current in control conditions (ICTR) (n = 6; ANOVA, Bonferroni). (c) Schematics of liquid perfusion protocol. Other annotations as in Figure 1 and Figure 6.