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. 2023 Jun 26;12(13):1723. doi: 10.3390/cells12131723

Figure 8.

Figure 8

TGN-20 or phloretin causes a distinct effect of HOC-evoked volemic changes in cultured hypothalamic astrocytes. (A–C). Fluorescence images of sulforhodamine 101 (SR101)-loaded astrocytic somata showing fluorescence intensity decrease, which report on relative volume increase (∆V/V0) during HOC under control conditions (CTR; (A)), or in the presence of 10 μmol/L of TGN-020 (B) or 30 μmol/L of phloretin (C) at different time points (a). The corresponding summarizing bar graphs showing average changes (b). TGN-020 or phloretin is applied at 1 min prior to HOC and then kept throughout HOC. Scale bar = 20 μm; CTR, n = 43; TGN-020, n = 14; phloretin, n = 54; **, p < 0.01; ***, p < 0.005, compared with 0 min HOC; ##, p < 0.01; ###, p < 0.005, compared with 1.5 min HOC; ANOVA, Dunn. (D) Summary of time lapse of volume changes showing distinct effects of TGN-020 (*, p < 0.05; **, p < 0.01; ***, p < 0.005 compared with CTR; ANOVA, Sidak) or phloretin (#, p < 0.05; ##, p < 0.01; ###, p < 0.005 compared with CTR; ANOVA, Sidak). Dotted line in extrapolation of the initial volume in CTR.