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. 2023 Jun 25;15(13):3341. doi: 10.3390/cancers15133341

Figure 2.

Figure 2

Vaccinia virus replication is attenuated in irradiated senescence-enriched human glioblastoma cell lines. (A) Experimental outline: human U87 and ED501 glioblastoma cell lines were either non-irradiated (0 Gy) or treated with a radiation dose of 10 Gy. Then, 7 days later, cells were infected with the indicated vaccinia viruses. Lysates were harvested at the indicated times and titered by plaque assay to assess virus yield. (B) Amplification of oncolytic ∆F4L∆J2R and wild-type (WT) vaccinia viruses in 0 Gy (non-irradiated; solid red lines) and 10 Gy (dashed blue lines) treated human U87 and ED501 glioblastoma cell lines. Cells were infected with 0.03 PFU per cell (left panels) or 3.0 PFU per cell (right panels). Data information: data represent three independent lysates titered in duplicate, mean ± SEM is shown. Graphs show fold change relative to lysates taken at t = 0. Significance determined by two-way ANOVA analysis (** = p < 0.01; *** = p < 0.001; **** = p < 0.0001).