Figure 6.

Radiation-induced senescence of human glioblastoma cells increases expression of NF-κB-associated genes, but not type I interferon related genes. Human U87 and ED501 glioblastoma cell lines were either non-irradiated (0 Gy) or treated with a radiation dose of 10 Gy. Then 7 days later, RNA was isolated and gene expression analyzed by RT-qPCR with 18S rRNA used for normalization. Shown is the increase in expression in 10 Gy treated relative to non-irradiated (0 Gy) glioblastoma cells of the following gene panels: (A) Type I interferon (IFN) related genes; (B) NF-κB-associated genes. Data information: data represent three independent experiments, mean ± SEM is shown. Asterisks indicate unpaired t-test comparing ΔCt values (* = p < 0.05; ** = p < 0.01; *** = p < 0.001; ΔCt values are shown in Figure S9).