Figure 1.

Spheroid formation for co-cultures of ASCs and cancer cells. (A) Schematic illustration of spheroid formation. Expansion of cells was performed in 2D before detachment and seeding in agarose micromolds. Cells were permitted to form spheroids over 48 h before harvest. (B, left) Silicone profile to cast micromolds. (B, middle) Agarose micromolds with spheroids after 48 h (256 spheroids/mold). Bar equals 1 cm. (B, right) Microscopic image of spheroids in micromolds after 48 h (1000 cells/spheroid; stromal cells and tumor cells 1:1). Bar equals 500 µm.