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. 2023 Jul 5;15(13):3501. doi: 10.3390/cancers15133501

Figure 2.

Figure 2

Characterization of mono- and co-culture spheroids of ASCs and MDA-MB-231 or MCF-7. (A) Mono- and co-culture spheroids of ASCs and MDA-MB-231 or MCF-7 after 48 h containing 1000 cells. Co-cultures of ASCs and breast cancer cell lines were pre-stained with PKH membrane dyes to visualize cell distribution (green: ASCs, red: cancer cells, blue: nuclei counterstained with DAPI). Bar equals 100 µm. (B) Spheroid compaction determined by measuring spheroid diameter of mono- and co-culture spheroids at different time points. (C) Shape of spheroids. Roundness was analyzed after 48 h using ImageJ, Version 1.53t. Data are presented as mean ± standard deviation (n = 80). Statistically significant differences (p < 0.05) are indicated by *.