These graphs depict the relative distinctness and similarities of all groups. A principal coordinates analysis (PCoA) diagram (A) shows groups overall similarity of using relative placement along axis PC1 and PC2. Dots in the PCoA plot represent an individual sample. Colors indicate group (Red = Sham vehicle, Blue = TBI vehicle, Green = Sham-FMT, and Purple = TBI-FMT). In the chart, sham and TBI groups are separated by the most distance representing the greatest level of between-groups-variability. Despite distance, these two groups show similar clustering representing the least amount of within-groups-variability of microbial phenotypes. The Atchison graph depicts the internal group variability (mean) using nodes (polygons) of large or small sizes to represent the level of internal group diversity. Distance of nodes from a central point (centroid) reveals group similarity of group means. This Atchison plot reveals that sham-FMT samples show the greatest within group diversity while the small TBI polygon depicts the least. Similar sized Sham and FMT-TBI nodes have similar levels of internal group diversity.