Skip to main content
. 2023 Jun 23;24(13):10542. doi: 10.3390/ijms241310542

Figure 1.

Figure 1

The effects of 7-oxysterols on CD14 expression at the mRNA and protein levels. THP-1 cells were serum-starved for 24 h in RPMI 1640 containing 0.1% endotoxin-free BSA, and then incubated with or without 7αOHChol, 7βOHChol, or 7K (5 μg/mL each) for the indicated periods. (A) CD14 transcript levels were assessed by qPCR. Y-axis values represent fold increases in CD14 mRNA levels normalized to GAPDH levels compared with those of control cells incubated without oxygenated cholesterol derivatives. Data are expressed as mean ± SD (n = 3 replicates/group). * p < 0.05 vs. control; *** p < 0.001 vs. control. (B) CD14 protein was detected by Western blot analysis, and (C) levels of mCD14 were measured by flow cytometry. The results are representative of three independent experiments. THP-1 cells were incubated with the indicated concentrations of 7αOHChol for 48 h after serum starvation. (D) The levels of CD14 transcripts were assessed by qPCR. Data are expressed as the mean ± SD (n = 3 replicates/group). ** p < 0.01 vs. control; *** p < 0.001 vs. control. (E) Levels of mCD14 were determined by flow cytometry. BSA: bovine serum albumin; 7αOHChol: 7α-hydroxycholesterol; 7βOHChol: 7β-hydroxycholesterol; 7K: 7-ketocholesterol; SD: standard deviation.