Skip to main content
. 2023 Jun 23;24(13):10527. doi: 10.3390/ijms241310527

Figure 1.

Figure 1

MC-LR-induced CRC cell–M2 macrophage interaction promoted CRC cell migration and analysis of relative proteins. The effect of MC-LR on the migration of (A) DLD-1 and (B) SW480 cells was determined by Transwell. The migration cells were stained with crystal violet, and the pictures were captured under light microscopy (×40). Secreted proteins were detected by iTRAQ quantitative proteomics technology. Samples were harvested from DLD-1 cell–M2 macrophage co-culture system with or without 25 nM MC-LR treatment for 48 h. (C) Differential expression proteins in co-culture system were colored based on the heat map scale (upregulated: red, FC ≥ 1.2; downregulated: blue, FC ≤ −1.2) by iTRAQ quantitative proteomics technology. IPA was used to analyze (D) the relationship between advanced malignant tumors and screened proteins, and (E) the interaction between TGF-β1 and related proteins. Red represents increased proteins; and green represents decreased proteins. (F) The proteins related to the migration of CRC cells were screened out. All data were presented as the mean ± SD from at least three independent experiments. ** p < 0.01, and *** p < 0.001, compared with the control group.